Bacterial lipid modification enhances immunoprophylaxis of filarial abundant larval transcript-2 protein in Mastomys model.
Identifieur interne : 003859 ( Main/Exploration ); précédent : 003858; suivant : 003860Bacterial lipid modification enhances immunoprophylaxis of filarial abundant larval transcript-2 protein in Mastomys model.
Auteurs : S. Sharmila [Inde] ; I. Christiana ; P. Kiran ; M V R. Reddy ; K. Sankaran ; P. KalirajSource :
- Parasite immunology [ 1365-3024 ] ; 2013.
Descripteurs français
- KwdFr :
- Activation des lymphocytes, Animaux, Anticorps antihelminthe (immunologie), Anticorps antihelminthe (sang), Antigènes d'helminthe (immunologie), Brugia malayi (immunologie), Cytokines (immunologie), Cytokines (métabolisme), Déterminants antigéniques des lymphocytes T (immunologie), Escherichia coli (génétique), Filariose lymphatique (), Filariose lymphatique (immunologie), Glycérides (immunologie), Humains, Immunisation, Ingénierie des protéines, Larve (immunologie), Lipides (immunologie), Lymphocytes T (immunologie), Murinae, Mâle, Protéines recombinantes (immunologie), Vaccins (immunologie).
- MESH :
- génétique : Escherichia coli.
- immunologie : Anticorps antihelminthe, Antigènes d'helminthe, Brugia malayi, Cytokines, Déterminants antigéniques des lymphocytes T, Filariose lymphatique, Glycérides, Larve, Lipides, Lymphocytes T, Protéines recombinantes, Vaccins.
- métabolisme : Cytokines.
- sang : Anticorps antihelminthe.
- Activation des lymphocytes, Animaux, Filariose lymphatique, Humains, Immunisation, Ingénierie des protéines, Murinae, Mâle.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antibodies, Helminth (immunology), Antigens, Helminth (immunology), Brugia malayi (immunology), Cytokines (immunology), Cytokines (metabolism), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Epitopes, T-Lymphocyte (immunology), Escherichia coli (genetics), Glycerides (immunology), Humans, Immunization, Larva (immunology), Lipids (immunology), Lymphocyte Activation, Male, Murinae, Protein Engineering, Recombinant Proteins (immunology), T-Lymphocytes (immunology), Vaccines (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth.
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth, Cytokines, Epitopes, T-Lymphocyte, Glycerides, Lipids, Recombinant Proteins, Vaccines.
- genetics : Escherichia coli.
- immunology : Brugia malayi, Elephantiasis, Filarial, Larva, T-Lymphocytes.
- chemical , metabolism : Cytokines.
- prevention & control : Elephantiasis, Filarial.
- Animals, Humans, Immunization, Lymphocyte Activation, Male, Murinae, Protein Engineering.
Abstract
As in many other parasitic diseases, efficacious vaccine for lymphatic filariasis has been elusive for want of new approaches leaving billions of people either debilitated or at risk. With multiple B- and T-cell epitopes, the abundant larval transcript-2 (ALT-2) of the filarial worm, Brugia malayi, has been shown to be a promising immunoprophylactic target. To enhance its efficacy, it was lipid modified using our recently developed protein engineering tool, which then offered 30% more immunoprotection (49 vs. 79%) in Mastomys coucha model. Sustained high levels of IFN-γ (about 100 times) and high antibody titres (10-fold) elicited by lipid-modified ALT-2, as compared to the native form, indicated the maintenance of Th1/Th2 balance that is impaired in filariasis. Thus, this study provides the basis for developing efficacious vaccines for filariasis and other parasitic diseases by exploiting bacterial lipid modification.
DOI: 10.1111/pim.12034
PubMed: 23495791
Affiliations:
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Kaliraj</name></author></analytic><series><title level="j">Parasite immunology</title><idno type="eISSN">1365-3024</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antibodies, Helminth (immunology)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (immunology)</term><term>Cytokines (immunology)</term><term>Cytokines (metabolism)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Escherichia coli (genetics)</term><term>Glycerides (immunology)</term><term>Humans</term><term>Immunization</term><term>Larva (immunology)</term><term>Lipids (immunology)</term><term>Lymphocyte Activation</term><term>Male</term><term>Murinae</term><term>Protein Engineering</term><term>Recombinant Proteins (immunology)</term><term>T-Lymphocytes (immunology)</term><term>Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia malayi (immunologie)</term><term>Cytokines (immunologie)</term><term>Cytokines (métabolisme)</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Escherichia coli (génétique)</term><term>Filariose lymphatique ()</term><term>Filariose lymphatique (immunologie)</term><term>Glycérides (immunologie)</term><term>Humains</term><term>Immunisation</term><term>Ingénierie des protéines</term><term>Larve (immunologie)</term><term>Lipides (immunologie)</term><term>Lymphocytes T (immunologie)</term><term>Murinae</term><term>Mâle</term><term>Protéines recombinantes (immunologie)</term><term>Vaccins (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term><term>Cytokines</term><term>Epitopes, T-Lymphocyte</term><term>Glycerides</term><term>Lipids</term><term>Recombinant Proteins</term><term>Vaccines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Cytokines</term><term>Déterminants antigéniques des lymphocytes T</term><term>Filariose lymphatique</term><term>Glycérides</term><term>Larve</term><term>Lipides</term><term>Lymphocytes T</term><term>Protéines recombinantes</term><term>Vaccins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Larva</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Immunization</term><term>Lymphocyte Activation</term><term>Male</term><term>Murinae</term><term>Protein Engineering</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Filariose lymphatique</term><term>Humains</term><term>Immunisation</term><term>Ingénierie des protéines</term><term>Murinae</term><term>Mâle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As in many other parasitic diseases, efficacious vaccine for lymphatic filariasis has been elusive for want of new approaches leaving billions of people either debilitated or at risk. With multiple B- and T-cell epitopes, the abundant larval transcript-2 (ALT-2) of the filarial worm, Brugia malayi, has been shown to be a promising immunoprophylactic target. To enhance its efficacy, it was lipid modified using our recently developed protein engineering tool, which then offered 30% more immunoprotection (49 vs. 79%) in Mastomys coucha model. Sustained high levels of IFN-γ (about 100 times) and high antibody titres (10-fold) elicited by lipid-modified ALT-2, as compared to the native form, indicated the maintenance of Th1/Th2 balance that is impaired in filariasis. Thus, this study provides the basis for developing efficacious vaccines for filariasis and other parasitic diseases by exploiting bacterial lipid modification.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Christiana, I" sort="Christiana, I" uniqKey="Christiana I" first="I" last="Christiana">I. Christiana</name><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Kiran, P" sort="Kiran, P" uniqKey="Kiran P" first="P" last="Kiran">P. Kiran</name><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name><name sortKey="Sankaran, K" sort="Sankaran, K" uniqKey="Sankaran K" first="K" last="Sankaran">K. Sankaran</name></noCountry><country name="Inde"><noRegion><name sortKey="Sharmila, S" sort="Sharmila, S" uniqKey="Sharmila S" first="S" last="Sharmila">S. Sharmila</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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